Pseudovirus (PV) belongs to the family of Pseudoviridae which consists of three genuses, Pseudovirus, Hemivirus, and Sirevirus. It is a viral genus that lacks certain virulence factors, making them significantly less dangerous to handle. Comparing to viable or attenuated viruses that mandate all operations to be performed in biosafety level (BSL) 3 and 4 laboratories, handling of Pseudovirus requires bio-contaminant level 2, which substantially enhanced its potential as an alternative to traditional methods.